S O Idowu 
,
S C Tambo,
A O Adegoke,
A A Olaniyi
For correspondence:- S Idowu Email: olakunleid@yahoo.com
Published: 24 June 2002
Citation: Idowu SO, Tambo SC, Adegoke AO, Olaniyi AA. Novelcolorimetric assay of mefenamic acid using 4-amino-3,5-dinitrobenzoic acid (ADBA). Trop J Pharm Res 2002; 1(1):15-22 doi: 10.4314/tjpr.v1i1.3
© 2002 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To develop a novel colorimetric assay method for mefenamic acid capsules.
Method: The new method (aromatic ring derivatization technique) is based on a diazo coupling reaction using diazotized 4-amino-3,5-dinitrobenzoic acid ADBA) as a chromogenic derivatizing reagent.
Result: Optimizationstudies showed that the coupling reaction is very fast and completed in less than 1 minute. A 1:1 drug to reagent stoichiometric ratio was obtained for the azo dye formed. The azo adduct formed exhibits bathochromic shift with absorption maximum (l max ) at 490 nm, which was selected as the analytical wavelength. Lower limit of quantitation of mefenamic acid was 1 m g/ml. The assays were linear over the concentration range of 1 - 6 m g/ml and reproducible. This new method has been successfully applied in the assay of mefenamic acid capsules with accuracy similar to the official (B.P) titrimetric method of assay (p>0.05) and has the advantages of speed, high sensitivity, lower limit of detection andcan be automated.
Conclusion: The method developed could find application in in-process quality control of mefenamic acid capsules.
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